Aims

To support the free and open dissemination of research findings and information on alcoholism and alcohol-related problems. To encourage open access to peer-reviewed articles free for all to view.

For full versions of posted research articles readers are encouraged to email requests for "electronic reprints" (text file, PDF files, FAX copies) to the corresponding or lead author, who is highlighted in the posting.

___________________________________________

Wednesday, November 26, 2008

Acetaldehyde stimulates monocyte adhesion in a P-selectin- and TNFα-dependent manner
Atherosclerosis Published online 26 Novewmber 2008

The aim of this study was to determine the effects of acetaldehyde on various steps of the monocyte recruitment cascade.

Acetaldehyde dose-dependently increased the number of CCR2 positive THP-1 monocytes, with a maximal increase of ∼50% observed in the presence of 10μM acetaldehyde. There was a significant increase in both the number of P-selectin positive cells and P-selectin receptor density when HUVEC were incubated with acetaldehyde. HUVEC TNFα mRNA expression and secretion were enhanced by acetaldehyde.

Moreover, acetaldehyde increased THP-1 and PBM adhesion to HUVEC. Inhibition of P-selectin or TNFα, using antibodies or siRNA-directed gene knockdown, attenuated acetaldehyde-induced monocyte adhesion.

In conclusion, acetaldehyde increased the number of CCR2 positive monocytes and stimulated endothelial cell P-selectin and TNFα expression. Moreover, acetaldehyde increased monocyte adhesion to endothelial cells, an effect that was both P-selectin- and TNFα-dependent.

These effects of acetaldehyde may contribute, in part, to the increase in coronary heart disease that is associated with binge patterns of alcohol consumption.

Read Full Abstract

Request Reprint E-Mail: john_cullen@urmc.rochester.edu
____________________________________________________________