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Wednesday, November 26, 2008

Acetaldehyde stimulates monocyte adhesion in a P-selectin- and TNFα-dependent manner
Atherosclerosis Published online 26 Novewmber 2008

The aim of this study was to determine the effects of acetaldehyde on various steps of the monocyte recruitment cascade.

Acetaldehyde dose-dependently increased the number of CCR2 positive THP-1 monocytes, with a maximal increase of ∼50% observed in the presence of 10μM acetaldehyde. There was a significant increase in both the number of P-selectin positive cells and P-selectin receptor density when HUVEC were incubated with acetaldehyde. HUVEC TNFα mRNA expression and secretion were enhanced by acetaldehyde.

Moreover, acetaldehyde increased THP-1 and PBM adhesion to HUVEC. Inhibition of P-selectin or TNFα, using antibodies or siRNA-directed gene knockdown, attenuated acetaldehyde-induced monocyte adhesion.

In conclusion, acetaldehyde increased the number of CCR2 positive monocytes and stimulated endothelial cell P-selectin and TNFα expression. Moreover, acetaldehyde increased monocyte adhesion to endothelial cells, an effect that was both P-selectin- and TNFα-dependent.

These effects of acetaldehyde may contribute, in part, to the increase in coronary heart disease that is associated with binge patterns of alcohol consumption.

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